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The NucleoCounter® NC-3000
from ChemoMetec

See Application Notes and Article

 Chemometec NC-3000

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Revolutionary instrument for advanced cell analyses

The NucleoCounter NC-3000 is a full-spectrum fluorescence microscope which provides precise automated detection, quantification and analysis of fluorescence at the single cell level.

Key Benefits:

• Excellent precision and reproducibility
• Extreme ease of use
• Fast analysis (very short total operation time)
• Automated data analysis and superior data visualization software
• Calibration, maintenance and service free
• All in One instrument with a small footprint
• Plug & Play System – preset parameter

Examples of applications:

Two-Step Cell Cycle
DNA Fragmentation
GFP Transfection Efficiency
Mitochondrial Potential
Vitality
Viability and Cell Count

True plug and play
The NC-3000 comes ready-to-use straight out of the box with predefined parameters. Just plug it into the power outlet, connect the computer and you are ready to analyse. NC-3000 comes with an intuitive software that allows you to perform sophisticated cellular analyses with just 3 easy clicks.

Choose between a wide variety of predefined assays
To aid the user in the day to day cell analyses the NC-3000 works on the principle of predefined assays with integrated collection, analysis and reporting of data that allows complex analyses to be run fully automated. All done in less than 1 minute

Avoid the trouble of doing complex post processing of your data
Image analysis, calculations and data reporting are performed automatically assuring superior and standardized results even with different users.



The NC-3000 offers:

• Excellent precision and reproducibility
• Extreme ease of use
• Fast and standardized analysis
• Direct cell counts
• No maintenance, no service, no calibration

Specifications:


Optics: Lens with x2 magnification, 1/2” CCD with 1360 x 1024 pixels

Sample device: NC-3000 cassettes NC-3000 disposable chamber slides (2, 4 and 8 chambers per slide)

Excitation (nm): Seven LED light sources with peaks at 365, 405, 455, 475, 500, 525 and 590 (standard-version)

Emission (nm): Eight interchangeable emission filters: 410-450, 415-525, 450-500, 500-555, 520-595, 555-605, 595-750, 630-1100 (standard version)

Sample consumption: 60 μl (cassettes) < 30 μl (chamber slides)

Analyzed volume: 1-4 μl sample (cassettes) 0.2-20 μl sample (chamber slides)

Optimal Range: 5 x 104 - 1 x 107 cells/ml

Cell types: Mammalian cells, yeast, insect cells, fish cells

Released applications: Cell count and viability, Transfection efficiency (GFP), Cell cycle analysis, Apoptosis Assays

PC Platform: Microsoft Windows 32 bit XP SP3, USB 2.0, Screen minimum 1024 x 768, recommended 2GB RAM

Analysis time: Typical 15-60 seconds (1 μl sample)

Data presentation: Images, tables, histograms, scatter plots

Weight: 14 kg

Dimensions: 29 x 29 x 31 cm [H x W x D]

Supply Voltage: 100-240V~ 50-60Hz

Power Consumption: 5/50W (power save mode/peak)

 


Application Notes and Article

Two-Step Cell Cycle

Cell Cycle of Fixed Cells

DNA Fragmentation

GFP Transfection Efficiency

Mitochondrial Potential

Vitality

Viability and Cell Count

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