Perform short-term experiments, initial feasibility tests, multi-level oxygen studies and dynamic oxygen cycling experiments.

The only system of its type that is a hermetically sealed container while allowing continuous control of oxygen. The Coy system results in less gas consumption (lower operating cost), and more reproducible oxygen results when compared to
tri-gas incubators and other cabinet systems.

• Hypoxic
• Normoxic
• Hyperoxic
• Physiologic
• Intermittent Hypoxia

O^₂ Control Cabinet System standard features:

• O^₂ controller and sensor (option available for ramp and cycle)
• All tubing and fittings to connect gas source to cabinet and controller(s)
• Pressure relief valve
• Two sensor ports
• Circulation fan
• Gas inlet
• Pull-out sliding shelf (multiple shelves for in vitro units)
• Sizes for tissue culture: four standard size units capable of handling nine to 72 plates
• Humidification tray for tissue culture units
• Sizes for live animal study: three standard sizes to meet your needs
• Custom-sized units available to meet specific lab needs

Coy’s modular designs and accessories, along with our 40 years of in-house customization experience, allow us to configure an economical cabinet for your lab.

How the Cabinet System Works

• Allows you to incubate samples (animal or tissue culture) quickly and conveniently at reduced or elevated O^₂ levels.
• The system offers the ability to change the O^₂ levels between multiple set points in increments of 0.1%.
• The microprocessor controls gas purges based on the sensor reading and user-adjustable set point.
• Depending on the application, you will need to supply one or all of the following gases: nitrogen, nitrogen with
  CO^₂ mix, and O^₂.
• The cabinet’s hermetic seal ensures that even with the controller(s) detached, you can maintain preset O^₂ levels depending on experimental conditions. This translates into less gas consumption compared to a semi-sealed cabinet.
• A ramp and soak upgrade is available as a factory-installed option for timed oxygen profile/cycle needs. The upgrade includes automatic cycling between multiple O^₂ set points plus voltage outputs that allow readings to transfer to a data logger, chart recorder or computer program. This is especially helpful for intermittent hypoxia experiments.

In vivo vs. in vitro Applications

• Animal units are designed around cage size, with custom sizing available.
• Waste removal can be handled by placing soda lime and/or activated carbon in a user-supplied containment tray.
  For a large number of animals or extended studies, the Coy Animal Filtration System is recommended. This system circulates the cabinet atmosphere through capsules of CarbO^₂Lime^® carbon dioxide absorbent and activated carbon to remove CO^₂, ammonia and other gases.
• Tissue culture units are designed to use existing lab incubators for temperature control while completely controlling oxygen levels (and optionally, CO^₂) inside the cabinet.
• The units enable long-term incubation and experiments with simultaneous O^₂ levels.
• Pull-out shelves provide easy access to samples.
  

Solving for CO^₂
The initial cost to purchase, versus cost of operation are your primary considerations in solving the CO^₂ dilemma.
There are two possible solutions.

• One is the Coy automatic CO^₂ control system, which provides constant digital monitoring and control of the CO^₂ in the cabinet and a separate CO^₂ tank.
• The other option is purchasing premixed tanks of gas for the O^₂ controller, which allows the proper amount of CO^₂ to be spiked into the system through the O^₂ controller. Purchasing premixed gas tanks will lower the initial cost of the cabinet system but may lead to higher operational cost depending on the gas demands of the experiments. In addition, premixed tanks do not provide constant feedback (and automatic correction) of the CO^₂ conditions inside the cabinet.

See the chart below for the various gas sources required based on cabinet use.

Pull-out shelves
The shelves give easy access to the unit. Shelf spacing is 1.5” for tissue culture units. There is a single shelf for cage support in live animal units.

Key Accessories

CO^₂ Control System

• Allows for precise control and monitoring of CO^₂ gases inside the glove box.
• A sensor relays information to the controller, which then opens the appropriate solenoid controlling a CO^₂ or N^₂ gas to equilibrate the CO^₂ levels based on the user-adjustable set point.
• The digital display allows the user to monitor the CO^₂ levels constantly, for 0-20% control in 0.1% increments.

Animal Filtration System

• Long-term animal containment will require the Coy Animal Filtration System to remove gaseous waste.
  These units can be automatic or manual.
• Lab personnel turn the manual filtration system off and on, based on lab protocols, while the automatic unit is governed by the CO^₂ setting.
• The animal filtration system is a closed-loop circuit with a pump that draws the glove box atmosphere out through
  the filters (CarbO^₂Lime^® and activated carbon) and returns it to the glove box.
  

O₂ Control Cabinet Sizes