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4300 DNA Analysis System

For the professional, student, or faculty researcher, the 4300 DNA Analysis System from LI-COR will accelerate your research and improve the quality of your data. High confidence in more precise data is the edge that researchers using LI-COR DNA analysis systems have had for more than 10 years.

Cat. No.
4300S: LIC-4300-01
4300L: LIC-4300-03

 

The Infrared Advantage:
4300 instrumentation, software, reagents, and applications are all part of an integrated system designed to achieve the highest accuracy. Infrared detection is a key advantage since biological contaminants, gels, and glass plates have little infrared fluorescence. WIth low background fluorescence, dye fluorescence signals are clean and strong. Infrared detection gives 4300 Systems excellent sensitivity.

Independent Detection
4300 Systems feature LI-COR's unique, two channel detection that eliminates errors due to fluorescence overlap. The fluorescence of the two IR dyes is separated by 100 nm. Two separate sets of optically tuned lasers and detectors measure fluorescence signals from IR dyes independently.

High Sensitivity and Wide Dynamic Range
Underloaded or overloaded samples are accurately detected because LI-COR images use more than 65,000 grayscales per pixel (16-bit) to display the wide dynamic range of the 4300 System. Unprocessed fluorescence data are captured in TIFF image format for highest fidelity.

 

 

Two Highly Reliable Diode Lasers:

Rugged and Reliable
The 4300 System is designed for multi-purpose operation. In addition to being an excellent research tool, the 4300 System is designed to meet demanding undergraduate training and research environments. Features such as diode lasers (similar to a CD player) make the instrument rugged and dramatically reduce maintenance and operation expenses. The instrument can also operate on demand after periods of inactivity without any expensive start-up costs.

Two Separate Detectors
As samples pass in front of the scanning laser/microscope, two solid-state avalanche photodiodes detect fluorescence. Optical filtering assures that each detector measures fluorescence from only one of the infrared dyes.

Two Real Time Images
A separate image (similar to an autoradiogram) for each IR dye is collected in real time and can be displayed in an Internet browser or LI-COR application software (e-Seq, Saga, etc.).

 

 

Easily Adapts to any Lab or Network:
The 4300 instrument contains a server that can operate either in a stand-alone configuration or connected to your network. An Internet browser or LI-COR Application Software can be used to start electrophoresis. The server software completely controls electrophoresis and data storage during a run. Two 16-bit TIFF images are stored on the 4300's server during a run.

The 4300 System gives you maximum data access. Any computer can connect to the 4300 System via Internet browser or LI-COR Application Software (e-Seq, Saga, etc.). In a network environment, image data can be accessed from your office or over the Internet. Secure, password-protected server software assures that only members of your project have access to your data.

The 4300 System is as expandable as the Internet. You can add all the capacity you'll ever need, without ever buying another computer. One computer can connect to any number of 4300 Systems to start runs or retrieve and analyze data.

Specifications:

Laser / Microscope
Laser Lifetime 40000 - 60000 hours typical
700 Channel Laser Source Solid-state diode laser at 685 nm
800 Channel Laser Source Solid-state diode laser at 785 nm
Detectors Two silicon avalanche photodiodes
Scanning Speed Five selectable scan speeds to optimize image quality
Electrophoresis Power Supply
Maximum Output 5000V, 40mA, and 120W
Operation Specifications
Operating Conditions 15 - 35C and dew point not greater than 19 C
Power Requirements Automatic voltage selection at 90-250 VAC and 47-63 Hz. 3 amps at 120volts; 380 watts maximum
Dimensions
4300S 59H x 51W x 47D cm (23" x 20"x 18"). 102 cm (40") wide with door fully open
4300L 84H x 51W x 47D cm (33" x 20"x 18"). 102 cm (40") wide with door fully open
Weight
4300S 29 kg (64 lb)
4300L 36.3 kg (80 lb)
Internal Server
Data Storage Capacity 80 GB for data storage and server software
Network Protocol TCP/IP
Network Connection RJ-45, 10/100 BaseT
Security Password protected access

 

Gel Electrophoresis Apparatus
Glass Height
Separation Distance
Resolution (bp)
Run Time (h)
Applications
66 cm*
56 cm
1000 - 1200
10
Sequencing
41 cm
31 cm
800
6
Sequencing
25 cm
15 cm
500
1.5 - 3
Genotyping and Sequencing
* Available on 4300L only.

 

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