• Potent Selection Agent in Molecular Biology Studies
• Effective Inhibitor of Eukaryotic Protein Synthesis

G418 is an aminoglycoside1-3 that is used as a selective agent in molecular genetics experiments4,5. It is toxic to bacteria, yeast, higher plant, mammalian cells and to protozoans and helminths. In general, this antibiotic is used for selection of transformants expressing an aminoglycosidemodifying enzyme. The genes are bacterial in derivation, but can be expressed in eukaryotic cells. Cells that harbor a gene conferring resistance to G418 are able to grow on media supplements with this antibiotic, making it an excellent agent for selection transfected cells.

References:

1. Weinstein, M.J., Wagman, G.H., Testa, R.T. and Marquez, J.A. (May 25, 1976) Antibiotic G-418 and the Production
   Thereof, U.S. Patent 3,959,254.
2. Weinstein, M.J., Wagman, G.H., Testa, R.T., Marquez, J.A. (December 14, 1976) Antibiotic G-418 and the Production
   thereof, U.S. Patent 3,997,403.
3. Jimenez, A. and Davies, J. (1980) Nature 287,869.
4. Davies, J. and Jiminez, A. (1980) J. Trop. Med. Hyg. 29(5) Suppl: 1089.
5. Ursic, D., Kemp, J.D. and Helgeson, J.P.(1981) Biochem, Biophys. Res. Comm. 101(3), 1031.

Note: see Chemicals & Reagents for complete alphabetical listing of AMRESCO® products and specifications