Take your analysis to a new level of sensitivity

SureBlue TMB Substrate
SureBlue ReserveTM provides maximum sensitivity for detection of peroxidase labeled conjugates in a microwell format and provides 50% more sensitivity than standard SureBlue TMB substrate. The substrate develops a deep blue color that is measurable at 650 nm and turns yellow when stopped by acidification and read at 450 nm. Single component, ready to use format of 3, 3', 5, 5'-Tetramethylbenzidine. No reagent preparation required, very convenient. It is stable for 2 years from date of manufacture when stored at 4°C.

TMB Substrate
Most sensitive chromogenic substrate for peroxidase detection, more sensitive than ABTS and OPD.
It develops a deep blue color in the presence of peroxidase-labeled conjugates measurable at 650 nm. Turns yellow when stopped by acidification and read at 450 nm. Recommended for ELISA. TMB can also be used in blotting applications with the purchase of the TMB Membrane Enhancer (50-77-01).
2-component format of 3, 3', 5, 5' - Tetramethylbenzidine, simply mix and use. Has longer shelf life than 1-component TMB substrates.

Features:

• Highest sensitivity: 50% more sensitive than SureBlue TMB in low end protein detection
• Excellent stability: 24 months from date of manufacture
• Recommended for both kinetic and endpoint ELISAs
• Working solution stable after stopping