Immunoprecipitation is a method by which a protein can be selectively purified from a complex mixture using an antibody in combination with a resin that binds the antibody. KPL's Immunoprecipitation Kits are designed to provide a versatile set of reagents, protease inhibitors, and Protein A Agarose that can be used for a variety of applications. Sufficient reagents are provided to lyse cells, solubilize and immunoprecipitate antigens from 20 x 1 mL cell aliquots with extra volume supplied for additional wash formulations.

Features and Benefits

• Flexibility: the content of the lysis buffer and wash solution can be modified to suit a specific protein.
• Protease Inhibitor Cocktail minimizes loss of target protein due to protease activity during lysis.
• All necessary reagents are conveniently supplied to perform immunoprecipitation (except for
  specific antibody), reducing time and expense in preparation.

Kit Contents

• Protein A Agarose or Protein G Agarose
• 0.5M Tris-HCl
• 1.5M NaCl
• 5% SDS
• 10% Sodium Deoxycholate
• 10% Triton X-100
• Protease Inhibitor Cocktail

Recommended Storage Conditions
Stable for at least 1 year when stored as recommended in kit manual.