- Simple, Fast and Ready-to-use Colorimetric Assay used to Detect Nitrite
- Sensitive Assay Detects Nitrite Concentrations from 20 to 100 µM
- Rapid Color Development Read at 540 nm
- Versatile - Can be performed in Cuvettes or Microplates
Nitric Oxide is detected in biological fluids via nitrite. Additionally, nitrate can be reduced to nitrite by NADPH in the presence of the enzyme Nitrate Reductase (NR). The nitrite formed reacts with sulfanilamide and N-(1-naphthyl)-ethylenediamine dihydrochloride instantaneously to give a red-violet diazo dye. The diazo dye is measured by its absorbance at 540 nm.
Product Description |
Size |
Cat. No. |
Nitrite Assay Kit
Includes:
Reagent 1
Reagent 2
Nitrite Standard
Contains suffi cient reagents to perform
1000 tests on a microplate. |
1 Kit |
AMR-N165-KIT |
Note: see Chemicals & Reagents for complete alphabetical listing of AMRESCO® products
and specifications |
