Protein Detector™ Western Blot Kits are designed for the colorimetric detection of proteins immobilized on membranes through either electrophoresis or dot blotting. Using the Protein Detector™ TMB Western Blot Kit, the combination of a highly specific, stable liquid conjugate with a sensitive chromogenic substrate allows rapid and accurate identification of samples. All solutions required for blocking and washing the membrane and for diluting antibodies are provided. For added flexibility, affinity purified
anti-mouse and anti-rabbit secondary antibodies conjugated with horseradish peroxidase are provided in stabilized, liquid form for quick dilution. TMB Membrane Peroxidase Substrate is provided as a convenient ready-to-use solution, which forms an insoluble blue precipitate when combined with the HRP-labeled antibody.

Product Features and Benefits

• Highest sensitivity of chromogenic substrates, protein detection as low as 50 pg.
• Very stable; one-component liquid substrate stable for one year.
• Ready-to-use reagents enable convenient and quick assay preparation.
• Anti-rabbit and anti-mouse antibodies supplied for versatility.

Kit Components: 
1. Detector Block Solution
2. Detector Block Powder
3. Wash Solution Concentrate
4. Peroxidase labeled Goat anti-Rabbit IgG (H+L)
    and Peroxidase labeled Goat anti-mouse IgG (H+L)
5. TMB Membrane Peroxidase Substrate

Recommended Storage Conditions:
Reagents are stable for 1 year when store at 2-8°C