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Applications of the Prominence HPLC Fluorescence Detector
HPLC systems are currently used in a wide variety of fields. Higher reliability in analysis data and higher efficiency in total analysis workflow are required for faster development of new drugs, for better food safety, and for meeting higher standards in environmental regulations.
Many analysis techniques use LCMS, which requires a front-end HPLC system to provide solvent delivery performance in the micro to semi-micro range and injectors with low sample carryover. Prominence is a network-ready HPLC system that meets the demands of today’s advanced users. Prominence features the world's first Web control, fastest sample injection, and highest detection sensitivity performance…functions that surpass current HPLC technology.
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A specially treated outer needle surface and improvement of needle seal composition greatly reduce sample adsorption. This performance is critical for achieving the high sensitivity of LC-MS/MS. In addition to that, the optional multi-liquid rinsing demonstrates almost undetectable sample carryover. The right chromatograms show the carryover of highly adsorptive chlorhexidine.
The SPD-20A detectors achieve the highest level of detection sensitivity and linearity by electronic noise elimination and a temperature-controlled flow cell. This performance is ideal for everyday needs and especially suitable for high-sensitivity analyses such as impurity quantitation.
With the SPD-20AV, both D2 and W lamps can be lit simultaneously so that high-sensitivity analysis for the full UV to VIS wavelength range is possible.
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Prominence features a wide variety of components, enabling you to build systems to meet any application need. Exceptional performance and functions such as high throughput and low sample carryover enhance any system from performing routine analysis to becoming a highly sophisticated LCMS front end.
The SIL-20 rack changer stores and transports up to 12 standard or deep-well microtiter plates to the Prominence autosampler for a significant advantage in sample capacity and throughput. Standard (ambient temperature) and cooler models with temperature control from 4 to 40oC are available.
Prominence will support multiple LC-20AB binary pumps, allowing an automated pretreatment system with column switching to use gradient elution in both sample pretreatment and analysis. With an additional pump, an automated system for direct serum injection with online sample dilution capability can be configured. |
Standard automated system
High-capacity system with rack changer |
Superior Component Performance
The world's first HPLC control by a web browser is available with the CBM-20A. Use any computer with Microsoft Internet Explorer 6.0 to control and monitor the Prominence HPLC system. Connection to the CBM-20A with a static IP address requires no special software to control and monitor HPLC systems on the network from the client PC. Easily check the status of networked HPLC systems, or monitor consumable parts for increased work efficiency.
Connection of HPLCs and PC workstations via the Ethernet enhances flexibility of instrument installation to efficiently use laboratory space, and provides a flexible analytical network environment by controlling access to the configured HPLC systems from client computers. Example of network environment using Prominence Networked Prominence systems support HPLC control, run monitoring, maintenance information, data management, and integrated analysis schedules.
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Applications of the Prominence HPLC
Fluorescence Detector
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HPLC control through Internet Explorer
Example of network environment using Prominence |
