Injection |
Mode: total loop or centered loop (except for 50-nL injection loop)
Carryover (with 100-nL internal loop): <0.055%, using rinsing parameters
Reproducibility (with 100-nL internal loop):
• total loop RSD: <2%
• centered loop RSD: <10%
Accuracy: ±5%
Valve switching speed: <0.2 seconds
Volume: 50 nL–100 µL with the standard injection valve (100-nL internal loop), 50 nL–500 nL with loop exchange (4-port valve), 2 µL–100 µL with loop exchange (6-port valve)
Available sample loops:
• internal: 50, 100, 200 or 500 nL
• external: 2, 5, 10, 20, 50 or 100 µL
Transfer flow rate: 2 µL/min (recommended); adjustable from 100 nL/min–100 µL/min |
Sampler Type |
Mobile sample platform cartesian robot |
Maximum Number of Sample Vessels/Bed |
For samples: 2 microplates (96- and 384-well, deep or shallow, or 1,536-well) or 112 x 2-mL vials; For reagents: 4 x 20-mL or 4 x 2-mL vial |
Probes(L x OD x ID) |
Probe options:
• sample prep (stationary): beveled-tip, stainless steel, grooved septum-piercing, constricted (103 x 1.5 x 0.4 mm)
• sample injection (stationary): beveled-tip, stainless steel, grooved septum-piercing, constricted (103 x 1.5 x 0.4 mm; tip OD: 0.7 mm) with fused silica tubing (263 x 0.36 x 0.1 mm)
• sample injection (stationary): flat tip for 1,536-well plates, stainless steel (103 x 2 x 0.4 mm; tip: 6 x 0.36 x 0.1 mm) with fused silica tubing (263 x 0.36 x 0.1 mm) |
Probe Rinse |
Inside probe rinse via dedicated syringe pump; outside probe rinse via peristaltic pump |
Tray Positioning Performance |
Accuracy: ± 0.05 mm (0.002 in.) in X/Y/Z dimensions |
Tray Speed Performance |
75 mm/sec. (3 in./sec) in X/Y dimensions; 50 mm/sec. (2 in./sec) in Z dimension |
Tray Cooling |
Sample cooling via external chiller |
Syringe Pumps |
Three syringe pumps for: high-precision injection, injection probe rinsing and sample preparation |
Reccommended Syringe Capacities |
Injection syringe: 10, 50, 100 or 500 µL
Rinsing syringe: 100, 500, 1000 or 2500 µL
Sample prep. syringe: 100, 500, 1000 or 2500 µL |
Available Valves |
Standard: one 4-port valve; optional: two additional 6-port valves |
Software |
Gilson 735 Sampler Software |
Communication Interface |
RS-232 or GSIOC; Three relay outputs, one 12V DC, 0.5A output, three contact inputs, and one analog signal input |
Front Panel |
Power, Detector Calibration, and Air Gap LED indicators; Detector, Dilutors, HP Valves, and Arm ERROR LED indicators; emergency STOP soft key |
Liquid Contact Materials |
PEEK, Teflon®, 316 stainless steel, fused silica |
Power Requirements |
Current rating: 3.15A
Frequency: 50–60 Hz
Voltage: 100–120V or 220–240V, mains voltage fluctuations not to exceed ±10% of the nominal voltage |
Environmental Operating Temperature |
5–40°C |
Manufacturing Standards |
Meets applicable Safety and EMC certification standards; UL and CE certified |
Dimensions (w x d x h) |
Sample platform: 74 x 54 x 39 cm (29 x 21 x 15 in.)
Hydraulic module: 41 x 25 x 23 cm (16 x 10 x 9 in.) |
Instrument Weight |
Sample platform: 47 kg (104 lbs.)
Hydraulic module: 10 kg (22 lbs.) |
Shipping Weight |
75 kg (165 lbs.) |
The 250 Nano Injector is designed to handle very small amounts of sample while maintaining good injection performance. This is accomplished by the injector’s innovative mechanical design—a mobile sample tray and stationary probes. An optical sensor is used to detect the air gap in front of the sample. This detection capability enables the injector to consume a minimum of sample and to position it precisely in the injection loop.
