Fast, reliable and safe media preparation
MEDIACLAVE 10 cat. no - TM-136010
MEDIACLAVE 30 cat. no - TM-136050
Features:
The MEDIACLAVE product range allows the rapid and gentle sterilisation of 1 – 30 L culture medium. Precise controlling and monitoring of temperature, time and pressure during the sterilisation process guarantee constant high quality. The intuitive graphical user interface and the simple programming make it very easy for everyone to operate the MEDIACLAVE.
First step - Medium formulation
The MEDIACLAVE is quickly set up. Insert the cuvette (container for media), fill in the coupling water (water jacket between the cuvette and the vessel for efficient heat transfer) and you are ready to prepare your culture medium.
Culture medium can be suspended and dissolved directly within the MEDIACLAVE. The strong magnetic stirrer guarantees homogeneous mixing within the vessel and prevents coagulation. Alternatively, culture medium can be dissolved and pre-swelled using the WATER BATH operation mode prior to sterilisation.
The intuitive, multilingual graphical user interface makes it very easy for everyone to use the MEDIACLAVE – no special training of the staff is required.
Up to 50 programs with user-defined parameter settings such as sterilisation temperature, sterilisation time or dispensing temperature
can be saved and recalled.
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Operation modes:
Two main operation modes for media preparation, plus two extra modes can be selected and the parameters set to your needs:
1. STANDARD mode: For the preparation of standard and highly sensitive culture media. Sterilisation temperature/time and dispensing temperature can be set.
2. CHOCOLATE AGAR mode: A special two-step program that allows the preparation of complex media. After the first sterilisation phase, supplement, e.g. blood, can be added through the adding port. Subsequently, a second heating phase will be performed.
3. WATER BATH mode: For pre-swelling culture media prior to sterilisation within the temperature range 30 – 80°C. In combination with an autoclave cuvette (only MEDIACLAVE 10), liquids in glassware can be thermostatted.
4. AUTOCLAVE mode (MEDIACLAVE 10 only): MEDIACLAVE 10, together with an optional autoclave kit can be used as a bench top autoclave for the sterilisation of small amounts of culture media in glassware such as Erlenmeyer flasks or test tubes |
| Second step - Medium sterilisation |
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High Operational Safety
An automatic tightness check at the beginning of each sterilisation cycle prevents the instrument from starting if, for example, the lid seal of the vessel is not inserted properly – avoiding incomplete sterilisation and subsequent media rejection. Furthermore, MEDIACLAVE is equipped with several independent monitoring systems for pressure and temperature, guaranteeing highest safety standards for the user and the working environment. The vessel lid is equipped with an autonomous overpressure safety valve and a burst disc in case all other electronic monitoring systems fail. |
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Fast Heating
Powerful heating elements permit fast media processing. This minimises thermal stress and ensures high fertility of your culture media. Pressure and temperature controlled deaeration guarantees saturated steam in the vessel – an absolute must for complete sterilisation. |
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Reliable Sterilisation
The large and strong magnetic stirrer with adjustable speed and reversing rotation direction ensures homogenous media preparation over a wide viscosity range. A Pt-1000 temperature probe and microprocessor control of all relevant process parameters allow reliable and complete sterilisation of culture media. |
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Rapid Cooling
Rapid cooling is enabled by an efficient plate heat exchanger. The spatial separation of the cooling water system and the sterilisation chamber makes it virtually impossible that culture media will get contaminated by cooling water. The built-in support pressure system prevents a delay in boiling during the rapid cool down phase and thereby allows an extraordinary fast and gentle cooling.
| Third step - Dispensing of the sterilised medium |
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Easy Dispensing
The wide adding port facilitates fast and safe addition of supplements such as antibiotics, blood or growth factors prior to dispensing. The automatic in-process sterilisation of the dispense port eliminates the risk of medium contamination during dispensing.
The vessel and the dispensing system of the MEDIACLAVE were designed to avoid dead volume, allowing laboratories to save costs due to maximal medium yield.
The MEDIACLAVE can be quickly and conveniently connected to the dispensing tubing of the automated Petri dish filler MEDIAJET, allowing the dispensing of agar media into up to 540 Petri dishes at the touch of a button.
In addition, the peristaltic pump DOSE IT can be used to fill containers of more unusual volumes or shapes, e.g. quadrangular Petri dishes, bottles or flasks.
Alternatively, large containers such as bottles can be filled by pressure dispensing. The optionally available pressure dispense kit, including a pinch valve box and a foot switch, allows the direct and fast dispensing of medium by automatically building up pressure inside the sterilisation chamber. |
Documentation of the media preparation process
The MEDIACLAVE provides all necessary tools to support individual needs in process documentation and validation. An integrated web server for direct connectivity via Ethernet allows remote monitoring of the sterilisation process and instrument parameters. Moreover, it allows the durable electronic storage of process data, circumventing the need to archive printouts. A digital signature according to FDA (21 CFR Part 11) / EU (GMP Annex 11) can be automatically added to the electronic process file.
If equipped with the optionally available dot matrix printer, high quality printouts insusceptible to fading can be archived. Printout intervals and parameters are individually adjustable for all process phases and hence allow laboratories to reduce paper consumption and operation costs. Alternatively, a standard external printer can be connected directly via the serial interface.
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Operational safety:
- New: A 4-stage safety program automatically monitors all pressure and heating functions of MediaClave. This guarantees 100% operating safety.
4-stage safety program:
Stage 1: Software check of all parameters entered
Stage 2: Pressure switches
Stage 3: Safety thermostats
Stage 4: Safety valves
Operating safety:
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Automatically opening lid
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Bayonet catch
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Excess pressure valve
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Opening for the addition of nutrients, inhibitors or other supplements
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Safety sensor
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Excess pressure sensor
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New: PT-100 sensor guarantees measurement accurate measurement during the process.
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Safety thermostats protects against overheating.
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Pressure manometer independent of the power supply
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Protection against contamination
Certificated safety:
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MediaClave is CE certified as safe and corresponds to the UL standard.
Cleaning:
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New:MediaClave incorporates an automatic self-cleaning program.
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New:The housing and membrane keyboard of MediaClave are particularly easy to clean.
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The stainless steel vessel can be easily removed for cleaning.
Specifications:
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MEDIACLAVE™ 10 |
MEDIACLAVE™30 |
| Capacity |
1-10L |
3-30L |
| Autoclave cuvette |
Ø 254 mm, H 203 mm |
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| Temperature range: |
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Sterilization |
70°C - 122°C |
70°C - 122°C
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Dispensing |
30°C - 75°C |
30°C - 75°C |
| Water Bath |
30°C - 80°C |
30°C - 80°C |
| Max. temperature deviation |
+1.0°C/-0.2°C |
+1.0°C/-0.2°C |
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Stirrer Speed Selectable |
50-200 rpm, reversing direction |
50-200 rpm, reversing direction |
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Heating capacity
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Max 3.0 kW |
Max 9.0 kW |
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Weight
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57kg |
85kg |
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Dimensions h x l x d
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480 x 550 x 640 mm |
1040 x 550 x 640 mm |
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Water connections
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3/4"
thread |
3/4"
thread |
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