|
Cyclo-Prep is a spin column-based kit for rapid
purification of plasmid DNA from 1-2 ml liquid cultures.
Cat. No. AMR-K179-50RXN
Features:
• Fast spin column format for efficient sample preparation
• DNA product compatible with automated fluorescent sequencing
• Phenol-free procedure reduces laboratory hazards
In just 15 minutes, up to 15 µg of ultra-pure plasmid DNA per ml of E. coli can be isolated using your tabletop microcentrifuge. The DNA isolated from Cyclo-Prep is
suitable for automated fluorescent sequencing, PCR, in vitro transcription, transformation and restriction enzyme digestion. The simple protocol provides an environmentally safe and
convenient alternative to common phenol-based DNA purification procedures. All reagents can be stored at room temperature and the protocol is easily performed using a pipettor and a
bench-top microcentrifuge.
|
Kit includes:
|
|
Resuspension Solution, 10 ml
|
|
Lysis Solution, 10 ml
|
|
Neutralizing Solution, 10 ml
|
|
Wash Buffer, 80 ml
|
|
Spin Columns, 50
|
|
Collection Tubes, 50
|
|
Contains sufficient reagents to perform 50 mini-preps
|
|