The Elu-Quik kit provides a convenient and versatile method for purifying DNA from 500 bp to 200 Kb. The kit is recommended for the isolation of genomic DNA from whole cells and tissues, as well as purification of single- and double-stranded fragments from gel slices or plasmid minipreps.

Features:

• Optimized buffer system provides high DNA yields
• Versatile kit allows isolation of DNA from a variety of sources
• Samples are eluted in TE or water, ready for further assays without ethanol precipitation
• Uniform glass rods minimize shearing of DNA

The Elu-Quik Kit relies on the affinity of DNA for glass particles in the presence of sodium perchlorate binding buffer. After several washing steps to remove contaminants and cellular debris, the DNA is eluted from the glass in TE or water. The highly pure DNA is ready for further experiments without the need for ethanol precipitation. The optimized buffers in the kit provide high yields, and unique rod-shaped glass particles reduce shearing of genomic DNA. Yields typically are greater than 650 µg from 10 8 cells.