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Detector™ RNA in vitro Transcription Biotinylation Kit

The Detector™ RNA in vitro Transcription Biotinylation Kit provides a method for synthesizing biotin-labeled RNA probes through incorporation of biotin dUTP during in vitro transcription. Strand specific probes may be generated using either T7 or SP6 RNA polymerase, which are included in the kit. The transcript reaction generates full length, single stranded RNA probes that can be used in a variety of applications, including Northern blotting, Southern blotting, and in situ hybridization.

Features and Benefits

  • Biotinylated RNA probes are stable for up to 1 year and can be reused many times.
  • RNA probes offer greater sensitivity than DNA probes on Northern blots.
  • β-actin positive control template provided to generate a control probe.
  • Biotinylated quantitation standard is provided to determine probe specific activity for greatest sensitivity without background in hybridization reactions.

Kit Contents
Sufficient reagents provided to perform 20 labeling reactions.

  1. 10X Ribonucleotide Labeling Mix
  2. 10X Transcription Buffer
  3. β-actin Template
  4. RNase Inhibitor
  5. SP6 RNA Polymerase
  6. T7 RNA Polymerase
  7. DNase I
  8. DEPC-treated Water
  9. Biotinylated Qunatitation Standard
  10. Spin-Pure Filters

Recommended Storage Conditions
Reagent is stable for 1 year when stored at -20°C.

Description

Size

Cat. No.

DetectorTM RNA in vitro
Transcription Biotinylation Kit

20 reactions

KP-60-01-02

 

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