The Protein Detector™ Western Blot Kit is designed for the colorimetric detection of proteins immobilized on membranes through either electrophoresis or dot blotting. The combination of highly specific mouse and rabbit secondary antibody-alkaline phosphatase conjugates with a sensitive chromogenic substrate allows rapid and accurate identification of samples. All solutions required for blocking and washing the membrane and for diluting antibodies are provided. For added flexibility, affinity purified anti-mouse and anti-rabbit secondary antibodies conjugated with alkaline phosphatase are provided in stabilized, liquid form for quick dilution. The BCIP/NBT substrate is provided as a convenient, ready-to-use solution, which forms an insoluble purple precipitate when reacted with the AP-labeled antibody.

Product Features and Benefits

• Ideal for routine colorimetric detection of overexpressed protein; sensitivity level to 500 pg.
• Very stable; one-component liquid substrate stable for one year.
• Ready-to-use reagents enable convenient and quick assay preparation.
• Anti-rabbit and anti-mouse antibodies supplied for versatility.

Kit Components:
1. Detector Block Solution
2. Detector Block Powder
3. Wash Solution Concentrate
4. Phosphatase labeled Goat Anti-Rabbit IgG (H+L)
    and Phosphatase labeled Goat Anti-Mouse IgG (H+L)
5. BCIP/NBT Phosphatase Substrate

Recommended Storage Conditions:
Reagents are stable for 1 year when store at 2-8°C