• Simple, Fast and Ready-to-use Colorimetric Assay used to Detect Inorganic Phosphate
• Sensitive Assay Detects Phosphate Concentrations from 2 to 20 µM
• Rapid Color Development Read at 620 nm
• Versatile - Can be performed in Cuvettes or Microplates

The Phosphate Assay Kit is based on the interaction between molybdate and malachite green dye.
The main principle involves the reaction of inorganic phosphate with molybdate to form a colorless unreduced phosphomolybdate complex which is converted to a blue colored complex when reduced under acidic conditions. Phosphomolybdate gives 20 or 30 times more color when complexed with malachite green.

The final product, a reduced green soluble complex, is measured by its absorbance at 620 nm and is a direct measure of inorganic phosphate in solution.

Note: see Chemicals & Reagents for complete alphabetical listing of AMRESCO^® products
and specifications