The LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multi-component kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer concentration, etc.).
The LavaLAMP™ DNA Master Mix greatly simplifies reaction optimization by limiting optimization to target specific components/conditions such as LAMP primer design, target concentration and reaction temperature. Additionally, this master mix is heat stable at 90°C for ≤5 minutes, which enables the addition of a reaction preheating step which may, depending on the target, increase assay sensitivity and decrease time to results.
Loop-mediated isothermal amplification (LAMP): Facilitates running amplification reactions in difficult test environments, enabling use outside of a high-tech laboratory.
Master Mix Format: Simplifies reaction setup and minimizes optimization required to produce the best assay results.
High Thermal Stability: Highly thermostable enzyme (up to 90°C for 5 min) enables preheating of reactions containing purified target DNA which, depending on the target, may increase assay sensitivity and decrease time to results.
Elevated LAMP Reaction Temperature (68-74°C): Improves primer specificity and reduces background amplification depending on the DNA target.
Freeze-dry Compatible: Enables generation of room temperature stable test kits through lyophilization.
