Secondary Antibodies & Conjugates

KPL pioneered the production of large-scale affinity purification and offers a wide range of antibodies with defined specificities. KPL’s polyclonal antibodies are obtained from the serum of goat or rabbit immunized with a particular antigen. The antiserum is affinity purified to remove nonspecific antibodies, increasing sensitivity and reducing background. Further purification may be required to remove potential nonspecific reactivities among related animal species or to reduce shared reactivity with other heavy and light chains. For example, antibodies affinity purified against mouse IgG may recognize similar regions on human IgG if it is present in the same immunoassay. Therefore, KPL further adsorbs antibodies to mouse immunoglobulins against immobilized human serum. 

LI-COR understand that quality and performance of secondary antibodies is crucial for Western Blotting, In-Cell Western assays, immunohistochemistry and many other applications. IRDye and VRDye secondary antibodies are highly cross-adsorbed, making them suitable for multi-colour detection. LI-COR and Mandel have come together to offer a wide array of secondary antibodies for a wide array of applications.