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Nexera HPLC and UHPLC Pumps
Shimadzu offers innovative pumps to address a wide range of separation needs. Accurate, reliable pump flow is critical to reproducible chromatography. Our intelligent pumps are equipped with auto-diagnostic and auto-recovery functions, avoiding mid-analysis failure (that can be caused by flow anomalies), reducing sample waste and minimizing downtime. Shimadzu leads the industry in providing more space and power saving designs among HPLC and UHPLC pumps.
Nexera HPLC/UHPLC Pump - LC-40B X3
Boost your separation with Shimadzu's most powerful UHPLC pump. Our goal is to ensure low pulsation, gradient formation accuracy, and retention time reproducibility with our parallel dual plunger design. One LC-40BX3 can deliver two solvents for reliable high-pressure gradient separation. Its new design has no flow channel meeting point inside the pump, making it a true binary pump in a compact housing. The ultra-high pressure tolerance (up to 130 MPa/1300 bar) allows for the use of sub-2μm particle columns.
LC-40B X3 Specifications
- Dual pump configuration, each with parallel dual micro plunger (10µL) construction, equivalent to 2 LC-40D X3 in a single, space-saving enclosure.
- Flow rate range from 0.1 uL/min to 10 mL/min with accuracy of ± 1 % or ± 2 µL/min (whichever is larger), and precision < 0.06% or 0.02 min SD (whichever is larger) of the set flow rate.
- Maximum pressure of 130 MPa/1300 bar (up to 3mL/min), with pH range of 1-14.
- Built-in peristaltic pump washes behind the seals to minimize salt precipitation if buffered mobile phases are used.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error and operation logs.
- FlowPilot automatic diagnostic and recovery capabilities allow monitoring of pressure fluctuations for anomalies, restoration of normal operation, and can also alert users of low solvent levels.
- Users can set solvent compressibility to further reduce pulsation during operation.
- External valve option to supply 2 different solvents to each pump.
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS software.
Nexera HPLC/UHPLC Pump - LC-40D X3
Discover faster analysis, better resolution, and more reproducible chromatography with our new flagship UHPLC pump. Our industry leading LC-20AD X3 pump ensures precision and accuracy at all flow rates. The ultra-high pressure capacity (up to 130 MPa/1300 bar) allows the of sub-2μm particle columns for high efficiency chromatography, especially for LC-MS/MS applications. An internal valve is available for solvent selection, low pressure gradient (LPGE) formation, and blending functions.
LC-40D X3 Specifications
- Parallel dual micro plunger (10 µL) construction for low pulsation and stable baseline.
- Flow rate range from 0.1 uL/min to 10 mL/min with accuracy of ± 1% (whichever is larger), and precision within < 0.06% or 0.02 min SD (whichever is larger) of the set flow rate.
- Operable in constant flow or constant pressure mode, as well as isocratic, binary high-pressure gradient, ternary high-pressure gradient, or quaternary low-pressure gradient mode.
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error and operation logs.
- Maximum pressure of 130 MPa/1300 bar (3mL/min) with pH range of 1-14.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- FlowPilot automatic diagnostic and recovery capabilities allow monitoring of pressure fluctuations for anomalies, restoration of normal operation, and can alert users of low solvent levels.
- Users can set solvent compressibility to further reduce pulsation during operation.
- Internal solvent selection valve (2 or 4-channel) or a low-pressure gradient formation (LPGE) unit can be added as options.
- Includes a seal wash kit to minimize salt precipitation if buffered mobile phases are used.
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS software.
Nexera HPLC/UHPLC Pump - LC-40D XS
Experience ultimate confidence with your resolution, sensitivity and separation with the innovative LC-40DXS UHPLC pump. The parallel dual plunger design provides the utmost in gradient accuracy and repeatability for UHPLC separations up to 105 MPa (1050 bar.) An internal LPGE unit can be added to enable low-pressure gradient formation and blending of mobile phases.
LC-40D XS Specifications
- Parallel dual micro plunger (10 µL) construction for low pulsation and stable baseline.
- Flow rate range of 0.1 uL/min to 10 µL/min with accuracy of ± 1% (whichever is larger), and precision < 0.06% or 0.02 min SD (whichever is larger) of the set flow rate.
- Operable in constant flow or constant pressure mode, as well as isocratic, binary high-pressure gradient, ternary high-pressure gradient, or quaternary low-pressure gradient mode.
- Maximum pressure of 105 MPa/1050 bar (up to 3 mL/min) with a pH range of 1-14.
- Internal solvent selection valve (2 or 4-channel) or a low-pressure gradient formation (LPGE) unit can be added as options.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- FlowPilot automatic diagnostic and recovery capabilities allow monitoring of pressure fluctuations for anomalies, restoration of normal operation, and can alert users of low solvent levels.
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error, and operation logs.
- Users can set solvent compressibility to further reduce pulsation during operation.
- Includes a seal wash kit to minimize salt precipitation if buffered mobile phase are used.
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS software.
Nexera HPLC/UHPLC Pump - LC-40B XR
Experience our LC-40B XR pump, a binary, high-pressure gradient solvent delivery unit that incorporates two pumps into one compact unit, with no compromises in gradient accuracy or retention time reproducibility. The space-saving design is an ideal choice for binary gradient separations up to 70 MPa (700 bar.)
LC-40B XR Specifications
- Dual pump configuration, each with parallel dual micro plunger (10 µL) construction, equivalent to 2 LC-40DXR in a single, space saving enclosure.
- Flow rate range from 0.1 uL/min to 10 mL/min with accuracy of ± 1 % or ± 2 µL/min (whichever is larger), and precision < 0.06% or 0.02 min SD (whichever is larger) of the set flow rate.
- Maximum pressure of 70 MPa/700 bar (up to 3mL/min) with pH range of 1-14.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- FlowPilot automatic diagnostic and recovery capabilities allow monitoring of pressure fluctuations for anomalies, restoration of normal operation, and can also alert users of low solvent levels
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error and operation logs
- Users can set solvent compressibility to further reduce pulsation during operation
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS software.
- External valve option to supply 2 different solvents to each pump.
- Includes a rinse kit to wash behind the seals to minimize salt precipitation if buffered mobile phases are used.
Nexera HPLC/UHPLC Pump - LC-40D XR
Discover the next generation of UHPLC pumps with extremely reliable and robust performance over an extended pressure tolerance (compared to traditional HPLC systems). The parallel dual plunger type design offers stable solvent delivery for a wide analytical flow rate range. Solvent selection, low pressure gradient (LPGE,) and blending functions can be used.
LC-40D XR Specifications
- Parallel dual micro plunger (10 µL) construction for low pulsation and stable baseline.
- Flow rate range of 0.1 uL/min to 10 mL/min with accuracy of ± 1 % or ± 2 µL/min (whichever is larger), and precision < 0.06% or 0.02 min SD (whichever is larger) of the set flow rate.
- Operable in constant flow or constant pressure mode, as well as isocratic, binary high-pressure gradient, ternary high-pressure gradient, or quaternary low-pressure gradient mode.
- Internal solvent selection valve (2 or 4-channel) or low-pressure gradient formation unit (LPGE) can be added as options.
- Maximum pressure of 70 MPa/700 bar (up to 3 mL/min) with a pH range of 1-14.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- FlowPilot automatic diagnostic and recovery capabilities allow monitoring of pressure fluctuations for anomalies, restoration of normal operation, and can alert users of low solvent levels.
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance error and operation logs.
- Users can set solvent compressibility to further reduce pulsation during operation.
- Includes a seal wash kit to minimize salt precipitation if buffered mobile phases are used.
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS control software.
HPLC Pump - LC-40D
Discover the efficient LC-40D pump for routine HPLC applications up to 44 MPa (440 bar.) The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. The dual piston design has minimal pulsation for stable baselines. Optional solvent selection valves can be added for solvent selection, low pressure gradient (LPGE) formation, and blending functions.
LC-40D Specifications
- Parallel dual micro plunger (10 µL) construction for low pulsation and stable baseline.
- Flow rate range from 0.1 uL/min to 10 mL/min with accuracy of ± 1 % or ± 2 µL/min (whichever is larger), and precision ≤0.06% RSD or 0.02 min SD of the set flow rate, (whichever is larger).
- Operable in constant flow or constant pressure mode, as well as isocratic, low pressure gradient, ternary high-pressure gradient, or quaternary low-pressure gradient mode.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error and operation logs.
- Maximum pressure of 44 MPa/440 bar (up to 5 mL/min) with pH range of 1-14.
- Operable in constant flow or constant pressure mode, as well as isocratic, binary high-pressure gradient, ternary high-pressure gradient or quaternary low-pressure gradient mode.
- Internal solvent selection valve (2 or 4-channel) or a low-pressure gradient formation unit (LPGE) can be added as options.
- A rinse kit can be added to wash behind the seals to minimize salt precipitation if buffered mobile phases are used.
- Users can set compressibility to further reduce pulsation during operation.
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS software.
Fully Unattended Operation from Startup to Shutdown
Nexera can be set to start up at a specified time, so that it can complete auto-purge, equilibration and baseline checks in advance, and be ready for analysis as soon as you arrive at the lab. Moreover, the system can be set up in advance to run without user intervention all the way from start up through analysis to shutdown.
You can view the status and predicted analysis completion time for multiple systems from any location via a smart device. None of these features requires any special software.
Auto-Diagnostics and Recovery
In rare cases, air bubbles can form in the mobile phase and cause problems if inhaled into the pump. Nexera has the ability to monitor baseline changes and pressure fluctuations to check for abnormalities.
When it detects an unusual fluctuation, it can automatically pause the analysis, purge the flow path, and restart analysis once it has confirmed recovery to normal pressure.
FlowPilot Protects Columns
HPLC columns can be damaged by sudden pump starts and stops or extreme gradient changes. The Nexera automatically uses FlowPilot (Smart Flow Control) to increase the flow rate gradually to the set point. There is no need to create startup protocols for each analysis.
(Pat. Pending)
Mobile Phase Levels Measured in Real Time
Reservoir tray weight sensors (optional) can be used to monitor the volume of mobile phase or autosampler rinse solution in up to twelve containers. The containers can also be checked remotely from a smart device.
You will no longer need to worry about running out of mobile phase mid-analysis, because the device will notify you before starting the run if the volume remaining is too low.
(Pat. Pending)
Space-Saving Design
The Nexera frees up bench space with a compact design two thirds the size of Shimadzu's previous model.
Energy-Saving Standby Mode
The Nexera uses over 70% less electricity when in standby mode, significantly reducing running costs and supporting an environmentally-friendly lab.
Easy-to-use Touch Panel
The touch panel screen and LabSolutions control screen display are uniform for easier operation.
Dual Injection Enables Simultaneous Analysis
Injection ports for two separate flow paths can be installed, allowing two different types of analysis (such as analysis of amino acids, organic acids or vitamins) to be performed using one system.
Ultra-low Carryover
The Nexera boasts ultra-low carryover, even on a high-sensitivity LC/MS/MS. This reduces time spent on rinsing, resulting in a shorter overall analysis time.
Add Samples Mid-Analysis
The autosampler allows the insertion of additional vials and MTPs even during analysis. The sample rack structure controls air flow inside the autosampler to maintain the internal temperature and prevent condensation.
High-Sensitivity Impurity Analysis
The SPD-M40 detector achieves an extremely high level of sensitivity and linearity (up to 2.5 AU). This allows quantitation of very lowconcentration impurities even in high-concentration samples. The UV cut-off filter installed in the detector prevents sample degradation due to UV light, helping to maintain good linearity at low concentrations.
Stable Baseline
Baseline fluctuations can affect peak area calculation, reducing the accuracy of quantitative results. The SPD-M40 photodiode array detector’s “Advanced TC-Optics” function adjusts the temperature of the flow cell, lamp, and optical system to lessen the impact of external temperature changes. Noise and drift have also been reduced by 40% compared to the previous model.
(Pat. Pending)
Analysis Cycle Time Less Than 10 Seconds
The SIL-40 autosampler can inject samples in as little as 5 seconds, twice as fast as the previous model. In addition, continuous analysis can be carried out on up to 44 MTPs (using 3 PLATE CHANGERS). Together these features dramatically increase analysis throughput. 8 injections can be completed within 1 minute (caffeine).
Pharmacokinetic analysis requires not only speed but also high reliability at low concentrations. With its ultra-fast injection and ultra-low carryover, the SIL-40 autosampler delivers high reproducibility and reliability, even during an ultra-fast 30-second analysis.
Automated Analysis of Thousands of Samples
Non-stop temperature-controlled analysis of thousands of samples is now possible with the SIL-40's optional plate changers. Set all your samples in advance with up to 14 MTPs or vial racks in each PLATE CHANGER. The autosampler's excellent temperature control also allows the insertion of additional vials and MTPs during analysis.
Quick and Reliable Mobile Phase Blending
The Nexera can automatically blend mobile phases at any set ratio. This speeds up the preparation of buffer solutions and the dilution of solvents, and can be used to easily prepare the exact amounts required for analysis, reducing waste as well as labour.
Simplified Method Development
The Nexera Method Scouting System is capable of automatically switching between combinations of up to 6 columns and 8 mobile phases. It can run unattended, maximizing available uptime. In addition, Method Scouting Solution Software can automatically create methods with different columns, mobile phases, and gradient conditions from a single base method.
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