The LavaLAMP™ DNA Component Kit is designed for loop-mediated isothermal amplification (LAMP) assay development. This kit contains a unique, highly processive DNA polymerase enzyme with strong strand displacement activity, making it well suited to LAMP and other isothermal amplification assays.
The enzyme in this kit is identical to the enzyme present in the LavaLAMP™ DNA Master Mix, but the individual reagent format of this kit, as opposed to the master mix format, gives you complete control of each LAMP assay and full optimization capabilities. Importantly, this kit is an exact deconstruction of the LavaLAMP® DNA Master Mix, and when reactions are setup using the initial Experimental reaction conditions outlined in the Component Kit User Manual, the composition of that reaction formulation matches the composition of the Master Mix-based reaction.
To aid in assay development and future lyophilization of LavaLAMP®-based reactions, all components in this kit are compatible with lyophilization. This feature means that reactions will not have to be reformulated or reoptimized to remove components that hinder lyophilization such as glycerol and betaine.
Loop-mediated Isothermal Amplification (LAMP): Allows researchers to run assays outside of high-tech laboratories using low complexity instrumentation
68-74°C Reaction Temperature: Reduces background amplification and improves primer specificity depending on the target
Lyophilization-ready: Component formulations enable generation of room temperature stable test kits through lyophilization
Custom/Bulk Available: Custom volumes and dispensing available to match your specific requirements
Matched Performance to LavaLAMP™ DNA Master Mix: Assists current users of the master mix product by streamlining additional assay optimization with this kit.
