The LavaLAMP™ RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which the RNA target is first converted into cDNA and then that cDNA acts as template for additional LAMP, all using a single DNA polymerase. By combining the strong activity of this enzyme with an optimized master mix formulation, we’ve produced an easy-to-use system that enables fast, sensitive detection of specific RNA targets.
Most LAMP enzymes are available as multi-component kits that require optimization of many parameters such as MgSO4, betaine, and enzyme concentrations as well as reaction temperature, primer design and primer concentration. The LavaLAMP™ RNA Master Mix greatly simplifies reaction optimization by providing a “pre-optimized reaction formulation” allowing you to focus your optimization on the two most important reaction parameters – RNA LAMP primer design and reaction temperature.
Isothermal Amplification: Facilitates running amplification reactions with simplified instrumentation
Master Mix Format: Streamlines reaction setup while reducing potential handling errors
Minimal Optimization: Focuses optimization on the two critical reaction parameters - primer design and reaction temperature
Lyophilization-ready: Avoids redesign of assays to remove components known to inhibit lyophilization – all components are lyophilization-compatible
