Like the related, LavaLAMP™ RNA Master Mix, this component kit is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. With these unique features, it is possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) using a single DNA polymerase. This enzyme first copies the RNA into cDNA, and then loop mediated isothermal amplification takes over to amplify the initial RNA target sequence present in the new cDNA making amplified and detectable target-specific, dsDNA.
The LavaLAMP™ RNA Component Kit is a deconstruction of the LavaLAMP™ RNA Master Mix, and as such, when reactions are setup using the initial Experimental reaction conditions outlined in the Component Kit User Manual, the composition of that reaction matches the composition of the recommended initial Master Mix reaction outline in its User Manual. The individual reagent format of this kit, as opposed to the master mix format, enables complete control of each LAMP assay allowing you to carefully identify the best reaction conditions for your specific target and primer set.
To aid in assay development and future lyophilization of LavaLAMP RNA-enzyme based reactions, all components in this kit are compatible with lyophilization. This feature means that reactions will not have to be reformulated or reoptimized after removal of components that prohibit lyophilization such as glycerol and betaine.
Isothermal Amplification: Enables running amplification reactions using less complex and lower cost instruments
Lyophilization-ready: All kit components are lyophilization compatible, thus avoiding redesign of assays to remove components known to inhibit lyophilization
Fully Optimizable: Non-master mix kit format gives you complete control of reaction formulation to maximize assay performance
Matched Performance to LavaLAMP™ RNA Master Mix: Assists current users of the master mix product by streamlining additional assay optimization with this kit.
