FLUOstar Omega microplate reader with combination optic for Absorbance, Fluorescence Intensity and Time-Resolved Fluorescence, including 8 filters. Automatic optic switching feature for detection mode changes and top/bottom reading. Endpoint and kinetic m

• High-speed absorbance measurements
• Measurements in up to 384-well plates
• Top and bottom reading
• Incubation up to 45 or 65 °C
• User-defined shaking
• Well scanning with up to 900 data points per well
• High-precision injectors with simultaneous reagent injection and detection
• Low-volume measurements

Detection Modes

• Fluorescence intensity - incl. FRET
• AlphaScreen®/AlphaLISA®
• Luminescence (flash and glow) - incl. BRET
• Time-resolved fluorescence
• TR-FRET
• UV/Vis absorbance spectra

Measurement Modes

• Top and bottom reading
• Endpoint and kinetic
• Sequential multi excitation
• Sequential multi emission
• Spectral scanning (absorbance)
• Ratiometric measurements
• Well scanning

Microplate Formats

• 6 to 384-well plates, user-definable
• LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier

• Robot compatible

Light Sources

• High energy xenon flash lamp

Detectors

• Low noise photomultiplier tube
• CCD spectrometer

Wavelength Selection

• Optical filters: ex and em wheels for 8 filters each
• UV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 sec / well

Optical Filters

• Excitation and emission filter wheels for 8 filters each

Optical Path Guides

• Top: liquid-filled light guides
• Bottom: fiber optics

Spectral Range

• Filters 240 - 740 nm or 240 - 900 nm for FI, FP, TRF
• 240 - 740 nm for LUM
• Spectrometer 220 - 1000 nm for ABS

Read Times

• Flying mode (1 flash)
• 9 s (96), 16 s (384)

Reagent Injection

• Up to 2 built-in reagent injectors
• Injection at measurement position (6 to 384-well)
• Individual injection volumes for each well (3 to 500 µL)
• Variable injection speed up to 420 µL / s
• Up to four injection events per well
• Reagent back flushing

Shaking

• Linear, orbital, and double-orbital with user-definable time and speed

Purge Gas Vent

• System to inject an atmosphere or to pull a vacuum into the reader

Incubation

• +4 °C above ambient up to 45 °C or 65 °C
• The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

Software

• Multi-user Reader Control and MARS Data Analysis Software included
• FDA 21 CFR Part 11 compliant

Dimensions

• Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg