Episcript RNase H- Reverse Transcriptase, 250,000 Units

• Produce full-length cDNA with RNAse H- mutant
• Achieve higher specificity at elevated temperatures, up to 55°C
• Part of the RapiDxFire 1-step RT-qPCR system for effective detection of viral RNA pathogens

Applications

• First-strand cDNA synthesis for subsequent PCR or real-time PCR.

Benefits

• Recombinant MMLV reverse transcriptase with greatly reduced RNase H activity
• Active at temperatures up to 55°C
• Highly efficient at producing full-length cDNA from as little as 50 pg of total RNA
• Best value in an RNase H- Reverse Transcriptase

Storage: Store only at -20°C in a freezer without a defrost cycle.

Storage Buffer: EpiScript RT is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 100 mM sodium chloride, 1 mM DTT, 0.1 mM EDTA, and 0.1% Triton® X-100.

Unit Definition: One unit of EpiScript RT catalyzes the incorporation of 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using saturating amounts of oligo(dT)-primed poly(rA) as template.

Contaminating Activity Assays: EpiScript RT is free of detectable exonuclease, endonuclease, and RNase activities.