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KASP™ is Biosearch Technologies' proprietary genotyping technology. The KASP genotyping reaction comprises three components:
The KASP Assay mix is specific to the SNP or InDel to be targeted and consists of two competitive, allele-specific forward primers and one common reverse primer. Each forward primer incorporates an additional tail sequence that corresponds with one of two universal FRET (fluorescent resonance energy transfer) cassettes present in the KASP-TF Master Mix. All custom KASP assays are available with the additional assay validation service.
KASP-TF Master Mix contains the two universal FRET cassettes (FAM and HEX), ROX™ passive reference dye, Taq polymerase, free nucleotides and MgCl2 in an optimised buffer solution.