Lucigen QuickExtract™ Plant DNA Extraction Solution; 50 mL (500 Extractions).

Rapid and efficient extraction of PCR-ready genomic DNA from plant leaf samples

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes (Fig. 1). Most leafy plants are suitable for DNA extraction using the QuickExtract Plant Solution, including Arabidopsis, barley, maize, emmer, pepper, rice, spelt, spinach, soybeans, and wheat (e.g., Fig. 2).

The QuickExtract Plant method allows for the inexpensive processing of one to hundreds of samples simultaneously, without grinding the sample, centrifugation, spin columns, or any toxic organic solvent. The procedure is fully compatible with robotic automation, provides a PCR-ready sample, and is reproducible (Fig. 3). Simply add the QuickExtract Plant solution to the sample and perform two sequential heating steps. A small aliquot of the sample is then used as a template for PCR or qPCR.

Fast: PCR-ready DNA in about 8 minutes for most samples
Simple: No bead-beating, freezing, or grinding of plant leaf material
Increased Yields: No centrifugation or spin columns used which reduce yields
Compatible: Extracted DNA is compatible with both real-time and endpoint PCR
Safe: Nontoxic reagets used throughout the procedure